Aller au menu Aller au contenu
Grenoble INP
Our engineering & Master degrees


School of engineering in Physics, Applied Physics, Electronics & Materials
Science

Our engineering & Master degrees

Labwork : Molecular biology & Biochemistry - 4PMBLMB6

A+Augmenter la taille du texteA-Réduire la taille du texteImprimer le documentTélécharger au format PDFEnvoyer cette page par mail Partagez cet article Facebook Twitter Linked In Google+ Viadeo
  • Number of hours

    • Laboratory works : 24.0
    ECTS : 3.0

Goals

The practicals are intended to illustrate the theoretical knowledge on DNA and proteins with biochemical experiments. They provide hands-on work with the basic techniques in molecular genetics and protein biochemistry.

Contact Marianne WEIDENHAUPT-BLACKLEDGE

Content

The practicals are organized in 3 sessions during wich a gene and its corresponding protein are analyzed.

  • The DNA sessions are focused on
    • plasmid DNA purification using alcaline lysis
    • quantification of DNA
    • sequence-specific DNA amplification using PCR
    • restriction analysis
  • The Protein sessions are focused on
    • expression of a recombinant protein in E coli
    • protein purification using affinity chromatography
    • protein quantification (Bradford)
    • SDS PAGE analysis and Western blot
    • quantification using ELISA


Prerequisites

Molecular Biology course, Semester 3 Biomedical Engineering

Tests

Semester 3 - The exam is given in english only 

Schedule
semester 3
SMPB2A

Written report



Additional Information

Semester 3 - This course is given in english only EN

Curriculum->BIOMED->Semester 3

A+Augmenter la taille du texteA-Réduire la taille du texteImprimer le documentTélécharger au format PDFEnvoyer cette page par mail Partagez cet article Facebook Twitter Linked In Google+ Viadeo

Written by Alexis Sableaux

Date of update June 11, 2015

Communauté Université Grenoble Alpes
×
To improve the quality of this site and the service rendered to the user, we use cookies audience measurement. By continuing your visit to this site, you agree to our use of cookies for this purpose. More