Aller au menu Aller au contenu
Our engineering & Master degrees


School of engineering in Physics, Applied Physics, Electronics & Materials
Science

Our engineering & Master degrees
Our engineering & Master degrees

> Studies > Engineering degree

Cell patterning lab

A+Augmenter la taille du texteA-Réduire la taille du texteImprimer le documentEnvoyer cette page par mail cet article Facebook Twitter Linked In
  • Number of hours

    • Laboratory works : 16.0
    ECTS : 1.0

Goals

Constraining cell adhesion to micrometric adhesive islands separated by antiadhesive
surfaces creates reproducible geometric cell shapes and controls cell physiology and
ultrastructure (spreading, proliferation and programmed cell death, division).

Contact Marc BLOCK, Herve GUILLOU

Content

Practical will be organized as follows:

  • Lithography of glass coverslips in clean room (0,5 day at CIME)
  • Cell culture and preparation of the structured supports (one day at IAB)
  • Cell staining observation and quantification under the microscope (one day at IAB)
    Photolithography is used on functionalized glass covers to pattern arrays of
    either adhesive (fibronectin)and anti-adhesive areas.
    When the lateral distance between two consecutive 4x4μm islands is 4 μm, NIH 3T3 cells
    attached and spread on the array in a manner similar to that usually observed on uniformly
    coated surfaces However when we increased the distance between adhesive islands to 8, 12
    or 16 μm, most of the cells adopted polygonal shapes which they attachedto the substratum
    solely by using adhesive spots at their corners.


Prerequisites

cell biology, microlithography techniques, surface functionalization

Tests

The exam is given in english only 

written report



Additional Information

This course is given in english only EN

Curriculum->BIOMED->Semester 5
Curriculum->Semesters A Choice->Semester 5
Curriculum->FSC-BIO->Semester 5

Bibliography

Thery M, Racine V, Pepin A, Piel M, Chen Y, Sibarita JB, Bornens M. (2005)The
extracellular matrix guides the orientation of the cell division axis. Nat Cell Biol.10:947-53.

A+Augmenter la taille du texteA-Réduire la taille du texteImprimer le documentEnvoyer cette page par mail cet article Facebook Twitter Linked In
Université Grenoble Alpes