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Number of hours
Laboratory works : 16.0
ECTS : 1.0
Constraining cell adhesion to micrometric adhesive islands separated by antiadhesive surfaces creates reproducible geometric cell shapes and controls cell physiology and ultrastructure (spreading, proliferation and programmed cell death, division).
Contact Marc BLOCK, Herve GUILLOU
Practical will be organized as follows:
Lithography of glass coverslips in clean room (0,5 day at CIME)
Cell culture and preparation of the structured supports (one day at IAB)
Cell staining observation and quantification under the microscope (one day at IAB) Photolithography is used on functionalized glass covers to pattern arrays of either adhesive (fibronectin)and anti-adhesive areas. When the lateral distance between two consecutive 4x4μm islands is 4 μm, NIH 3T3 cells attached and spread on the array in a manner similar to that usually observed on uniformly coated surfaces However when we increased the distance between adhesive islands to 8, 12 or 16 μm, most of the cells adopted polygonal shapes which they attachedto the substratum solely by using adhesive spots at their corners.