Number of hours
- Laboratory works 16.0
ECTS
ECTS 1.0
Goal(s)
Constraining cell adhesion to micrometric adhesive islands separated by antiadhesive
surfaces creates reproducible geometric cell shapes and controls cell physiology and
ultrastructure (spreading, proliferation and programmed cell death, division).
Content(s)
Practical will be organized as follows:
- Lithography of glass coverslips in clean room (0,5 day at CIME)
- Cell culture and preparation of the structured supports (one day at IAB)
- Cell staining observation and quantification under the microscope (one day at IAB)
Photolithography is used on functionalized glass covers to pattern arrays of
either adhesive (fibronectin)and anti-adhesive areas.
When the lateral distance between two consecutive 4x4μm islands is 4 μm, NIH 3T3 cells
attached and spread on the array in a manner similar to that usually observed on uniformly
coated surfaces However when we increased the distance between adhesive islands to 8, 12
or 16 μm, most of the cells adopted polygonal shapes which they attachedto the substratum
solely by using adhesive spots at their corners.
Prerequisites
cell biology, microlithography techniques, surface functionalization
Test
The exam is given in english only
written report
Additional Information
This course is given in english only
Course list
Curriculum->BIOMED->Semester 5
Curriculum->Semesters A Choice->Semester 5
Curriculum->FSC-BIO->Semester 5
Bibliography
Thery M, Racine V, Pepin A, Piel M, Chen Y, Sibarita JB, Bornens M. (2005)The
extracellular matrix guides the orientation of the cell division axis. Nat Cell Biol.10:947-53.