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Our engineering & Master degrees

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DNA microarray lab

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  • Number of hours

    • Laboratory works : 12.0
    ECTS : 1.0

Goals

To prepare, characterize and use a hybridation DNA microarray
To understand some notions important in biotechnology : specificity, reproducibility, sensitivity and confidence

Contact Franz BRUCKERT, Marianne WEIDENHAUPT-BLACKLEDGE

Content

Phase 1 : DNA extraction, amplification and labeling with a fluorophore. Quantification.

Phase 2 : spotting of oligonucleotides probes with a microarrayer

Phase 3 : hybridization with fluorescent target DNA and washings

Phase 4 : quantification of immobilized fluorescence and statistical analysis



Prerequisites

Molecular biology
Biosensors and microarrays

Tests

Report under the form of a scientific article (4 pages)



Additional Information

Curriculum->BIOMED->Semester 5
Curriculum->Semesters A Choice->Semester 5
Curriculum->FSC-BIO->Semester 5

Bibliography

P. Soularue et X. Gidrol 2002 : Les puces à ADN techniques de l’ingénieur RE 06-2002

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Université Grenoble Alpes