Number of hours
- Laboratory works 12.0
ECTS
ECTS 1.0
Goal(s)
To prepare, characterize and use a hybridation DNA microarray
To understand some notions important in biotechnology : specificity, reproducibility, sensitivity and confidence
Content(s)
Phase 1 : DNA extraction, amplification and labeling with a fluorophore. Quantification.
Phase 2 : spotting of oligonucleotides probes with a microarrayer
Phase 3 : hybridization with fluorescent target DNA and washings
Phase 4 : quantification of immobilized fluorescence and statistical analysis
Prerequisites
Molecular biology
Biosensors and microarrays
Test
Report under the form of a scientific article (4 pages)
Additional Information
Course list
Curriculum->BIOMED->Semester 5
Curriculum->Semesters A Choice->Semester 5
Curriculum->FSC-BIO->Semester 5
Bibliography
P. Soularue et X. Gidrol 2002 : Les puces à ADN techniques de l’ingénieur RE 06-2002
French State controlled diploma conferring a Master's degree
