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Labwork : Molecular biology & Biochemistry - 4PMBTP19

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  • Number of hours

    • Laboratory works : 24.0
    ECTS : 3.0

Goals

The practicals are intended to illustrate the theoretical knowledge on DNA and proteins with biochemical experiments. They provide hands-on work with the basic techniques in molecular genetics and protein biochemistry.

Contact Marianne WEIDENHAUPT-BLACKLEDGE

Content

The practicals are organized in 5 sessions during wich a gene and its corresponding protein are analyzed.

  • The DNA sessions are focused on
    • plasmid DNA purification using alcaline lysis
    • quantification of DNA
    • sequence-specific DNA amplification using PCR
    • restriction analysis
  • The Protein sessions are focused on
    • expression of a recombinant protein in E coli
    • protein purification using affinity chromatography
    • protein quantification (Bradford)
    • SDS PAGE analysis and Western blot
    • quantification using ELISA


Prerequisites

Tests

The exam is given in english only 

Schedule
semester 3
SMPB2A

Written report



Additional Information

This course is given in english only EN

Curriculum->BIOMED->Semester 3

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Université Grenoble Alpes