Labwork : Molecular biology & Biochemistry - 4PMBTP19

Goal(s)

The practicals are intended to illustrate the theoretical knowledge on DNA and proteins with biochemical experiments. They provide hands-on work with the basic techniques in molecular genetics and protein biochemistry.

Content(s)

The practicals are organized in 5 sessions during wich a gene and its corresponding protein are analyzed.

• The DNA sessions are focused on
  • plasmid DNA purification using alcaline lysis
  • quantification of DNA
  • sequence-specific DNA amplification using PCR
  • restriction analysis

• The Protein sessions are focused on
  • expression of a recombinant protein in E coli
  • protein purification using affinity chromatography
  • protein quantification (Bradford)
  • SDS PAGE analysis and Western blot
  • quantification using ELISA