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Molecules of life_DNA chips - 4PMTMBI2

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  • Number of hours

    • Lectures : 8.0
    • Tutorials : 8.0
    • Laboratory works : 8.0
    ECTS : 2.0
Content

MOLECULES OF LIFE :

  • Structure and function of proteins, case studies of different proteins illustrate their diverse functions
  • Structure, organization and function of nucleic acids (DNA and RNA); in particular genome organization and gene regulation will be discussed; RNA molecule functions will be highlighted
  • Enzyme catalysis, the Michaelis and Menten model for enzyme catalysis, structural and functional analysis of a protease
  • Selected topics of interest chosen with the students: examples are fluorescent proteins, the molecular biology of vision, the human genome....

Practicals : DNA chips

  • Phase 1 : Surface functionalisation of a glass slide and covalent grafting of DNA probes
  • Phase 2 and 3 : Hybridation with fluorescent target DNA and detection/quantification of fluorescence

Equipment used: Liquid spotting robot and fluorescence scanner

Teaching type: practical lab work : 8 Hours



Prerequisites

None

The course Molecules of life is linked to the Biochip practicals

The course Practicals : DNA chips is linked to the Molecules of life

Tests

The exam is given in english only 

Making mode : written report



Additional Information

This course is given in english only EN

Curriculum->NANOTECH->Semester 4

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Université Grenoble Alpes