Number of hours
- Laboratory works 24.0
ECTS
ECTS 3.0
Goal(s)
The practicals are intended to illustrate the theoretical knowledge on DNA and proteins with biochemical experiments. They provide hands-on work with the basic techniques in molecular genetics and protein biochemistry.
Contact Marianne WEIDENHAUPT-BLACKLEDGEContent(s)
The practicals are organized in 5 sessions during wich a gene and its corresponding protein are analyzed.
- The DNA sessions are focused on
- plasmid DNA purification using alcaline lysis
- quantification of DNA
- sequence-specific DNA amplification using PCR
- restriction analysis
- The Protein sessions are focused on
- expression of a recombinant protein in E coli
- protein purification using affinity chromatography
- protein quantification (Bradford)
- SDS PAGE analysis and Western blot
- quantification using ELISA
Prerequisites
Test
The exam is given in english only
Schedule
semester 3
SMPB2A
Written report
Additional Information
This course is given in english only
Course list
Curriculum->BIOMED->Semester 3