Phelma Formation 2022

Cell biology labwork - 4PMBCBP9

  • Number of hours

    • Lectures 0
    • Projects 0
    • Tutorials 0
    • Internship 0
    • Laboratory works 8.0
    • Written tests 0

    ECTS

    ECTS 1.0

Goal(s)

In these practicals the student learns eucaryote cell culture techniques. Qualitative and quantitative analysis methods to evaluate cellular function are taught.

Contact Franz BRUCKERT

Content(s)

During these practicals 2 main experiments are proposed: Transfection and Intracellular pH assessment

1. Transfection: Eucaryote HeLa cells are cultivated for this experiment. A transitory transfection is done using plasmids that allow fluorescence localisation in the cell using targeted proteins or organelles. Transfected cells are observed and analysed using fluorescence microscopy. Images are aquired and interpreted.
2. Intracellular pH: Intracellular pH is assessed using yeast expressing a pH sensitive fluorescent protein (pHluorin), targeted either in the cytosol or in the mitochondria. In addition, a membrane permeant fluorophore is used to measure intravacuolar pH. A calibration curve is established. The cell responses to glucose addition is investigated, in the presence of various inhibitors. Quantitative data are recorded and analyzed to measure intracellular pH gradients, in relation to cell physiology.



Prerequisites

Basics in molecular and cell biology

Test

Semester 8 - The exam is given in english only 

written and oral report
The written report consists in a scientific poster for each labwork
There is no second session



session 1 condition normale : 100% Rapport écrit

Pas de session 2

session 1 condition confinement :

Additional Information

This course brings 0.5 ECTS to students in Block Specialisation course NBS

Semester 8 - This course is given in english only EN
Course list
Curriculum->Engineering degree->Semester 8
Curriculum->Master->Semester 8