Cell biology labwork - 4PMBCBP9
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Number of hours
- Lectures : 0
- Tutorials : 0
- Laboratory works : 8.0
- Projects : 0
- Internship : 0
- Written tests : 0
ECTS : 1.0
Goals
In these practicals the student learns eucaryote cell culture techniques. Qualitative and quantitative analysis methods to evaluate cellular function are taught.
Contact Marianne WEIDENHAUPT-BLACKLEDGE
Content During these practcals 2 main experiments are proposed: Transfection and Cell viability assessment
Transfection: Eucaryote HeLa cells are cultivated for this experiment. A transitory transfection is done using plasmids that allow fluorescence localisation in the cell using targeted proteins or organelles. Transfected cells are observed and analysed using fluorescence microscopy. Images are aquired and interpreted.
Cell Viability: Cell viability is assessed using yeast and a respiratory inhibitor. The effect of different parameters is investigated (concentration, time, ...) and the survival of cells is tested using fluorescent indicators of cell function (MitoTracker, DAF, IP, AO). Quantitative data are recorded and images are taken using fluorescence micrsocopy.
PrerequisitesBasics in molecular and cell biology
Tests Semester 8 - The exam is given in english only 
written or oral report
Additional Information This course brings 0.5 ECTS to students in Block Specialisation course NBS
Semester 8 - This course is given in english only

Curriculum->Engineering degree->Semester 8
Curriculum->Master->Semester 8
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Date of update March 13, 2019