Cell biology labwork - 4PMBCBP9

During these practcals 2 main experiments are proposed: Transfection and Cell viability assessment

Transfection: Eucaryote HeLa cells are cultivated for this experiment. A transitory transfection is done using plasmids that allow fluorescence localisation in the cell using targeted proteins or organelles. Transfected cells are observed and analysed using fluorescence microscopy. Images are aquired and interpreted.
Cell Viability: Cell viability is assessed using yeast and a respiratory inhibitor. The effect of different parameters is investigated (concentration, time, ...) and the survival of cells is tested using fluorescent indicators of cell function (MitoTracker, DAF, IP, AO). Quantitative data are recorded and images are taken using fluorescence micrsocopy.

Basics in molecular and cell biology

Semester 8 - The exam is given in english only 

written or oral report

This course brings 0.5 ECTS to students in Block Specialisation course NBS