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Labwork : Molecular biology & Biochemistry - 4PMBLMB6

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Number of hours
- Lectures : 0
- Tutorials : 0
- Laboratory works : 24.0
- Projects : 0
- Internship : 0
- Written tests : 0
ECTS : 3.0

Goals

The practicals are intended to illustrate the theoretical knowledge on DNA and proteins with biochemical experiments. They provide hands-on work with the basic techniques in molecular genetics and protein biochemistry.

Contact Marianne WEIDENHAUPT-BLACKLEDGE

Content

The practicals are organized in 3 sessions during wich a gene and its corresponding protein are analyzed.

The DNA sessions are focused on
- plasmid DNA purification using alcaline lysis
- quantification of DNA
- sequence-specific DNA amplification using PCR
- restriction analysis

The Protein sessions are focused on
- expression of a recombinant protein in E coli
- protein purification using affinity chromatography
- protein quantification (Bradford)
- SDS PAGE analysis and Western blot
- quantification using ELISA

Prerequisites

Molecular Biology course, Semester 7 Biomedical Engineering

Tests

Semester 7 - The exam is given in english only