Number of hours
- Lectures 0
- Projects 0
- Tutorials 0
- Internship 0
- Laboratory works 24.0
- Written tests 0
ECTS
ECTS 3.0
Goal(s)
The practicals are intended to illustrate the theoretical knowledge on DNA and proteins with biochemical experiments. They provide hands-on work with the basic techniques in molecular genetics and protein biochemistry.
Contact Marianne WEIDENHAUPT-BLACKLEDGEContent(s)
The practicals are organized in 3 sessions during wich a gene and its corresponding protein are analyzed.
- The DNA sessions are focused on
- plasmid DNA purification using alcaline lysis
- quantification of DNA
- sequence-specific DNA amplification using PCR
- restriction analysis
- The Protein sessions are focused on
- expression of a recombinant protein in E coli
- protein purification using affinity chromatography
- protein quantification (Bradford)
- SDS PAGE analysis and Western blot
- quantification using ELISA
Prerequisites
Molecular Biology course, Semester 7 Biomedical Engineering
Test
Semester 7 - The exam is given in english only 
Face-to-face SESSION1:
Type of assessment: Written report
Not session 2
session 1 Rapport de TP
session 2: pas de session 2
session1confiné: pas de TP; travail bibliographique
session2 confiné: travail bibliographique
Additional Information
Semester 7 - This course is given in english only
Curriculum->Engineering degree->Semester 7