Molecular biology Labwork - 4PMBBMB2

Informations générales

  • Number of hours

    • Lectures 0
    • Projects 0
    • Tutorials 0
    • Internship 0
    • Laboratory works 20.0
    • Written tests 0

    ECTS

    ECTS 0.0

Goal(s)

The practicals are intended to illustrate the theoretical knowledge on DNA and proteins with biochemical experiments. They provide hands-on work with the basic techniques in molecular genetics and protein biochemistry.

Contact Marianne WEIDENHAUPT

Content(s)

The practicals are organized in 3 sessions during wich a gene and its corresponding protein are analyzed.

The DNA sessions are focused on
plasmid DNA purification using alcaline lysis
quantification of DNA
sequence-specific DNA amplification using PCR
restriction analysis

The Protein sessions are focused on
expression of a recombinant protein in E coli
protein purification using affinity chromatography
protein quantification (Bradford)
SDS PAGE analysis and Western blot



Prerequisites

Molecular Biology course, Semester 7 Biomedical Engineering

Test

Semester 7 - The exam is given in english only 



Additional Information

Semester 7 - This course is given in english only EN

Course list
Curriculum->BIOMED->Semester 7