During these practcals 2 main experiments are proposed: Transfection and Cell viability assessment
Transfection: Eucaryote HeLa cells are cultivated for this experiment. A transitory transfection is done using plasmids that allow fluorescence localisation in the cell using targeted proteins or organelles. Transfected cells are observed and analysed using fluorescence microscopy. Images are aquired and interpreted. Cell Viability: Cell viability is assessed using yeast and a respiratory inhibitor. The effect of different parameters is investigated (concentration, time, ...) and the survival of cells is tested using fluorescent indicators of cell function (MitoTracker, DAF, IP, AO). Quantitative data are recorded and images are taken using fluorescence micrsocopy.